Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PBRM1

Cell type

Cell type Class
Muscle
Cell type
RH-4
NA
NA

Attributes by original data submitter

Sample

source_name
RH4
cell line
RH4
diagnosis
rhabdomyosarcoma
treatment
4 hours treatment with 0.2% DMSO (Sigma, D8418)
genotype
wild type
antibody
anti-PBRM1, Active Motif, 61381

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After reverse crosslinking, ChIP DNA was purified using MinElute PCR Purification Kit (Qiagen). ChIPseq Library

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
36346592
Reads aligned (%)
57.2
Duplicates removed (%)
46.7
Number of peaks
12729 (qval < 1E-05)

hg19

Number of total reads
36346592
Reads aligned (%)
56.5
Duplicates removed (%)
48.4
Number of peaks
12504 (qval < 1E-05)

Base call quality data from DBCLS SRA